Genetic Variation within Native Populations of Endemic Silkmoth Antheraea assamensis (Helfer) from Northeast India Indicates Need for In Situ Conservation

نویسندگان

  • Y. Tunginba Singh
  • Sudeshna Mazumdar-Leighton
  • Mahaswetta Saikia
  • Prashant Pant
  • Sochanngam Kashung
  • Kartik Neog
  • Rajen Chakravorty
  • Suresh Nair
  • Javaregowda Nagaraju
  • Cheerukeri Raghavendra Babu
چکیده

A. assamensis is a phytophagous Lepidoptera from Northeast India reared on host trees of Lauraceae family for its characteristic cocoon silk. Source of these cocoons are domesticated farm stocks that crash frequently and/or wild insect populations that provide new cultures. The need to reduce dependence on wild populations for cocoons necessitates assessment of genetic diversity in cultivated and wild populations. Molecular markers based on PCR of Inter-simple sequence repeats (ISSR) and simple sequence repeats (SSR) were used with four populations of wild insects and eleven populations of cultivated insects. Wild populations had high genetic diversity estimates (H(i) = 0.25; H(S) = 0.28; H(E) = 0.42) and at least one population contained private alleles. Both marker systems indicated that genetic variability within populations examined was significantly high. Among cultivated populations, insects of the Upper Assam region (H(i) = 0.19; H(S) = 0.18; H(E) = 0) were genetically distinct (F(ST) = 0.38 with both marker systems) from insects of Lower Assam (H(i) =0.24; H(S) =0.25; H(E) = 0.3). Sequencing of polymorphic amplicons suggested transposition as a mechanism for maintaining genomic diversity. Implications for conservation of native populations in the wild and preserving in-farm diversity are discussed.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Genetic Diversity and Population Structure of Indian Golden Silkmoth (Antheraea assama)

BACKGROUND The Indian golden saturniid silkmoth (Antheraea assama), popularly known as muga silkmoth, is a semi-domesticated silk producing insect confined to a narrow habitat range of the northeastern region of India. Owing to the prevailing socio-political problems, the muga silkworm habitats in the northeastern region have not been accessible hampering the phylogeography studies of this rare...

متن کامل

External Morphology (chorion) of the Eggs of Muga Silkworm Antheraea Assamensis (helfer) after Treated with Juvenile Hormone -iii and 20 Hydroxyecdysion

*Central Muga Eri Research & Training Institute, Lahdoigarh, Jorhat-785700, Assam,India Regional Medical Research Centre (ICMR), Lahoal, Dibrugarh-785001, Assam,India Department of Life Sciences, Dibrugarh University, Dibrugarh786400, Assam,India ABSTRACT: The surface of the chorion of the golden silk producing muga silkworm, Antheraea assamensis,Helfer is covered with polygons in network patte...

متن کامل

The mitochondrial genome of Muga silkworm (Antheraea assamensis) and its comparative analysis with other lepidopteran insects

Muga (Antheraea assamensis) is an economically important silkmoth endemic to the states of Assam and Meghalaya in India and is the producer of the strongest known commercial silk. However, there is a scarcity of genomic and proteomic data for understanding the organism at a molecular level. Our present study is on decoding the complete mitochondrial genome (mitogenome) of A. assamensis using ne...

متن کامل

Microsatellite markers for the Indian golden silkmoth, Antheraea assama (Saturniidae: Lepidoptera).

Antheraea assama, an economically important and scientifically unexplored Indian wild silkmoth, is unique among saturniid moths. For this species, a total of 87 microsatellite markers was derived from 35 000 expressed sequence tags and a microsatellite-enriched sub-genomic library. Forty individuals collected from Tura and West Garo Hills region of Northeast India were screened for each of thes...

متن کامل

Molecular diversity within and between Ajowan (Carum copticum L.) populations based on inter simple sequence repeat (ISSR) markers

Study of genetic relationships is a prerequisite for plant breeding activities as well as for conservation of genetic resources. In the present study, genetic diversity among and within 15 Iranian native Ajowan(Carum copticum L.) populations were determined using inter simple sequence repeat (ISSR) markers. Twelve selected primers produced 153 discernible bands, with 93 (60.78%) being ...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:

دوره 7  شماره 

صفحات  -

تاریخ انتشار 2012